Identification of skin-migrated DDCs and LCs in human SLN. (A) Average mean fluorescence intensity (MFI) values for CD1a expression levels on CD11c^hiCD1a^int and CD11c^intCD1a^hi SLN cDC (n = 14) (B) Gating strategy for CD11c^hiCD1a^int and CD11c^intCD1a^hi DCs, migrated from human skin explants (data from one representative experiment of 4 is shown). (C) Percentages positive cells (of SLN leukocytes, open bars: left y-axis) and the median fluorescence intensities (MFI, closed bars: right y-axis) for intracellular DC-SIGN, langerin, and E-cadherin on CD11c^hiCD1a^int and CD11c^intCD1a^hi cDC in SLN single-cell suspensions (left, n = 3) and skin-migrated cells (right, n = 4). Bars represent the mean ± SEM; dotted line indicates MFI = 2; *P < .05 in paired 2-sided Student t test.