Expression of BDCA3 and CD103 on SLN cDC subsets. Percentages positive cells (left) and MFI levels (right) of (A) BDCA3/CD141 (n = 11) and (B) CD103 (n = 6) surface expression on the 4 cDC subsets identified in human SLNs. *P < .05 in 1-way ANOVA with post hoc multiple comparison Tukey test. Numbers indicate the significant difference compared with (1) CD11c^hiCD1a^int, (2) CD11c^intCD1a^hi, (3) CD11c⁺CD1a⁻CD14⁻, and (4) CD11c⁺CD1a⁻CD14⁺ subsets. (C) Histogram plots of one representative experiment showing expression levels of BDCA3/CD141 (top) and CD103 (bottom) on SLN cDC subsets. The markers were placed based on respective isotype control staining. (D) Characterization of the localization and morphology of BDCA3/CD141⁺ cells within SLN imprint samples. Shown is a CD3 staining to identify the paracortical T-cell areas (left, magnification ×400) as well as BDCA3 staining in 2 magnifications (middle, ×400 and right, ×630).