T-cell stimulatory ability of the CD1a⁻ and CD1a⁺ SLN cDC subsets. T-cell stimulatory abilities of human SLN cDC subsets were analyzed by allogeneic MLR with CFSE-labeled human T cells. Data from one representative experiment of 3 performed are shown in panels A and in B the average percentage of proliferated cells (n = 3, left y-axis) combined with IFNγ present in day 7 MLR supernatants (right y-axis) are shown. Correlations between the frequencies of SLN cDC subsets and (C) IL-6, IL-1β, and IL-12p70 present in 24-hour unstimulated cultures of SLN single-cell suspensions (n = 7) or (D) IFNγ, IL-2, and IL-4 in 24-hour supernatants of SLN T cells stimulated by anti-CD3 and anti-CD28 (n = 5). Correlations were determined by use of the Pearson r test. Differences were considered significant when P < .05.