L-Hcy–increased IK1 expression and oxidation/nitration of SKs and IKs in HCMECs. HCMECs were treated with or without L-Hcy at the indicated concentrations for 48 hours. Western blot analysis was performed for SK3 and IK1 in HCMECs extracts before (A) and after (D) immunoprecipitation with 3-NT antibody. Values are means ± SEM (n = 3). *P < .05 compared with no-treatment parallel control; †P < .05 compared with Hcy 200μM; and #P < .05 compared with Hcy 500μM. CT indicates the control group; UA, uric acid.