IL-7R signaling and complete Ig gene rearrangements. (A-E) The levels of V_H-DJ_H, Vκ-Jκ, Vκ-Kde, IntronRSS-Kde, and Vλ-Jλ rearrangements were quantified as in Figure 2 in FACS-purified CD127⁺ and CD127⁻ populations from cultures in which endogenous IL-7 was neutralized for either 4 or 7 days. Each bar represents the mean ± SD of triplicate experiments except CD127⁺ (0 days), which represents quadruplicate values (the triplicate values from Figure 2 plus 1 additional experiment donor matched to one of the experiments in which IL-7 was neutralized).