CD70TG mice develop IFN-γ–dependent anemia. Analysis of WT, CD70TG, IFN-γ^−/−, and CD70TG*IFN-γ^−/− mice. (A) Hemocytometric analysis at 4 and 12 weeks of age: Hgb indicates hemoglobin; and Hct, hematocrit. Data are mean ± SD bar graphs for 3-5 mice per group. *Significant difference (P < .05) between CD70TG mice and all other groups (2-way ANOVA with Bonferroni correction). (B-C) Representative dot plots and bar graph depicting the percentage of blood reticulocytes (ie, CD71⁺ and thiazole orange-positive cells). (D) Spleens. (E) Spleen weight. (F) Representative dot plots of the splenic erythroid compartment. Numbers I, II, III, and IV refer to the respective erythroblast subsets defined by Socolovsky et al.²²  (G) Absolute numbers of indicated erythroblast subsets per spleen. Data are mean ± SD in bar graphs for 3 mice per group. Results are representative from 3 independently performed experiments. *Significant difference (P < .05) between CD70TG mice and all other groups (1-way ANOVA). (H) Plasma EPO levels. Data are mean ± SD in bar graphs for 5-8 mice per group. *Significant difference (P < .05) between CD70TG mice and all other groups (1-way ANOVA with Bonferroni correction). (B-H) Mice were used in the age range of 10-16 weeks old.