IFN-γ production in CD70TG mice inhibits erythroid BM output. (A) Absolute numbers of various erythroblast subsets or (B) relative contribution of pro-erythroblasts (percentage Ery I [Ter119^medCD71^high] from all erythroid [Ter119^med/high cells]) in BM (isolated from 2 femurs and 2 tibiae) of WT, CD70TG, IFN-γ^−/−, and CD70TG*IFN-γ^−/− mice. (C-D) BFU-e and CFU-e numbers of unfractionated BM from WT, CD70TG, IFN-γ^−/−, and CD70TG*IFN-γ^−/− mice. (E) Colony assay of FACS-sorted CMPs from WT and CD70TG mice. (F-G) Absolute numbers of IFN-γ–producing CD4 and CD8 T cells in BM of WT and CD70TG mice, measured after phorbol myristate acetate/ionomycin stimulation. (H-I) Effect of IFN-γ on the in vitro BFU-e and CFU-e colony-forming capacity of BM cells from WT mice. Data are mean ± SD for 3 mice per group (A-D,F-G) or triplicate analysis (E,H-I). Results are representative from 3 (A-D) or 2 (E-I) independently performed experiments. *P < .01 (A) or P < .05 (B-H) using 1-way ANOVA with Bonferroni correction (A-D) or nonpaired Student t test (E-I).