P17 induces the adhesion, migration, and transmigration of isolated human monocytes. (A) Static adhesion assay on fibrinogen. Monocytes were stimulated for 2 minutes at 37°C with PBS (NT), GST, fMLP, or p17 at the indicated concentrations. Bars represent the means ± SD of 3 independent experiments performed in triplicate. Statistical analysis was performed by 1-way ANOVA and the Bonferroni posttest was used to compare data. ***P < .001 compared with NT. (B) Transwell migration assay of monocytes in response to PBS (NT), GST, fMLP, or p17 at the indicated concentrations. Bars represent the means ± SD of 3 independent experiments performed in duplicate. Statistical analysis was performed by 1-way ANOVA and the Bonferroni posttest was used to compare data. **P < .01 and ***P < .01 compared with NT. (C) Static adhesion assay on HUVECs. Monocytes were stimulated for 30 minutes at 37°C with PBS (NT), GST, fMLP, or p17. Bars represent the means ± SD of 4 independent experiments performed in duplicate. Statistical analysis was performed by paired 2-tail Student t test. *P < .05 and ***P < .001 compared with NT. (D) Transwell migration assay of monocytes through HUVEC monolayer in response to GST, fMLP, or p17. Bars represent the means ± SD of 3 independent experiments performed in duplicate. Statistical analysis was performed by paired 2-tail Student t test. ***P < .01 compared with NT. (E-H) Serial sections obtained from PBS-injected (left panels) or p17-injected (right panels) mice and stained for H&E (E-F) or anti-Iba1 (G-H). Skin biopsies obtained from p17-injected mice contain a greater number of infiltrating cells compared with mice injected with PBS. Most of the infiltrating cells correspond to large mononuclear cells (F insert), as defined by the expression of the monocyte/macrophage marker Iba1 (H). Scale bar indicates 200 μm.