CXCR1 expression in Jurkat cells is sufficient to mediate chemotaxis in response to p17. (A) Surface expression of CXCR1 on Jurkat cells transfected with the control vector pEGFP-N3 or with pEGFP-N3 expressing CXCR1. Cells were incubated with isotype control mAb (solid histogram) or mAb to CXCR1 (open histogram), and then stained with APC-conjugated secondary Ab and analyzed by flow cytometry. (B-C) Transwell migration assays of Jurkat cells transfected with pEGFP-N3 or pEGFP-N3-CXCR1 in response to the indicated treatments. Bars represent the means ± SD of 3 independent experiments performed in duplicate. Statistical analysis was performed by 2-way ANOVA. The Bonferroni posttest was used to compare data. **P < .01; ***P < .001. (B) Transfected Jurkat cells were stimulated for 90 minutes at 37°C with PBS (NT), IL-8, GST, or p17. (C) Jurkat cells, pre-incubated for 30 minutes at 37°C with 50 μg/mL of Ctrl mAb or mAb to p17 (MBS-3), were subsequently stimulated for 90 minutes at 37°C with PBS (NT), IL-8, GST, or p17.