P17 exerts some IL-8–like activities on neutrophils. (A) Transwell migration assay of neutrophils in response to the indicated concentration of p17. IL-8 was used as a positive control and GST as an irrelevant protein. Bars represent the means ± SD of 3 independent experiments performed in duplicate. Statistical analysis was performed by 1-way ANOVA, and the Bonferroni posttest was used to compare data. ***P < .001 compared with NT. (B) Priming effect of p17 on fMLP-stimulated neutrophil oxidative burst. Cells were untreated or treated with IL-8, p17, or GST for 15 minutes at 37°C, and then stimulated or not with fMLP (100nM). Results are expressed as nanomoles of O2− production/2 × 105 cells. Results are representative of 4 different experiments with similar results.
