GATA-3 inhibits the generation of adult B-lymphoid cells. (A) Peripheral blood was analyzed from primary recipient (1°-, top) and secondary recipient (2°-, bottom) mice by flow cytometry every 4 weeks for 16 weeks after transplantation. Ratios of donor-derived (CD45.2⁺CD45.1⁻) B-lymphoid (CD19⁺) to myeloid (Gr1⁺Mac1⁺) cells in peripheral blood from Gata3^+/+ (+/+, ●) or Gata3^z/z (z/z, ▵) cells are shown. Data represent the summary of 5 independent experiments with an average of 11 recipient mice for 1°- and 2 independent experiments with an average of 40 recipient mice for 2°- in each group with SEM. *P < .03. (B) The absolute numbers of donor-derived cells (CD45.2⁺CD45.1⁻) in myeloid (Gr1⁺Mac1⁺, left panels), B-lymphoid (CD19⁺B220⁺, middle panels), and T-lymphoid (Thy1.2⁺CD3⁺, right panels) populations per mouse (2 femurs plus 2 tibias) from +/+ or z/z donor cells in BM. Chimerism was analyzed from 1°- (top panels) and 2°- (bottom panels) recipient mice 16-20 weeks after transplantation. Data represent the summary of 3 independent experiments with an average of 12 recipient mice for 1°- and 2 independent experiments with an average of 20 recipient mice for 2°- in each group with SEM. *P < .03. NS indicates not significant.