GATA-3 participates in a transcription network governing the HSC cell cycle. (A) qRT-PCR analysis of GATA-2 mRNA relative expression in BM LSK (top panel) and LSK CD150⁺CD48⁻CD34⁻ (bottom panel) populations from adult Gata3^flox/flox [f/f(−)], Gata3^flox/+:Tg^Mx1Cre [f/+(+)], and Gata3^flox/flox:Tg^Mx1Cre [f/f(+)] 3 weeks after poly(I:C) treatment. Data represent the summary of at least 3 mice of each genotype from 3 independent experiments with SEM. NS indicates not significant. (B) Relative expression of various transcription factors (horizontal axis) in BM LSK (top panel) and LSK CD150⁺CD48⁻CD34⁻ (bottom panel) cells from adult f/f(−) (black bars), f/+(+) (gray bars), and f/f(+) (white bars) 3 weeks after poly(I:C) treatment. The mRNA expression level was normalized to that of f/f(−) in each set. Data represent the summary of 5 mice from 3 independent experiments of each genotype with SEM. *P < .05. NS indicates not significant.