Bβ15-42 peptide protects mice from renal I/R injury. Male C57Bl/6 mice were subjected to 27 minutes of bilateral renal I/R injury or sham surgery and 3.6 mg/kg Bβ15-42 peptide or random peptide was administered intravenously 1 minute after reperfusion. (A) The medullary congestion after ischemia was significantly less in the Bβ15-42 peptide–administered mice compared with mice administered random peptide. Outline of vascular congestion traced by white dots. (B) SCr and BUN as indicators of renal dysfunction and urinary levels of Fg and Kim-1 as indicators of kidney injury were measured at 24 and 48 hours in all the groups (n = 5/group of sham, n = 10/group at 24 hours, and n = 5/ group at 48 hours). *P < .05 as determined by 1-way ANOVA in comparison with sham mice. (C) Representative H&E stained kidney sections comparing sham, Bβ15-42 and random peptide–administered groups of mice at 24 and 48 hours after ischemia. Bar represents 100 μm (20× magnification).
