Bβ15-42 peptide aids in the resolution of injury by decreasing necrosis/apoptosis and inducing rapid tissue regeneration. Paraffin-embedded kidneys of mice subjected to 27 minutes of bilateral I/R that were administered either Bβ15-42 or random peptides were compared at 24 and 48 hours for number of apoptotic cells (green; A) by TUNEL assay (bar represents 100 μm, 40× magnification). Arrowheads indicate TUNEL-positive nuclei. (B) Number of proliferative cells determined by Ki67-positive staining cells (red; bar represents 50 μm, 60× magnification). The numbers of positive staining TUNEL and Ki67 nuclei are represented graphically on the right of respective photomicrographs. Arrowheads indicate Ki67 positively stained nuclei. *P < .05 as determined by Student t test between the 2 groups within the same time point.
