Effect of lactadherin on PCA of coincubated target APL cells and phagocytes. Target APL cells were preincubated with 2nM lactadherin or annexin V for 10 minutes at room temperature. Clotting time (A), intrinsic FXa (B), extrinsic FXa (C), and thrombin (D) of 1 × 106 target APL cells, or 1 × 106 target APL cells opsonized by annexin V or lactadherin, or incubation of 1 × 106 target cells with phagocytes (THP-1–derived MΦs or HUVECs) for 2 hours, or incubation of 1 × 106 annexin V–opsonized or lactadherin-opsonized target cells with phagocytes for 2 hours were determined. Lactadherin and phagocytes cooperatively increased coagulation time and reduced enzyme complexes of target APL cells. *P < .05 compared with single target APL cells. +P < .05; #P < .01; and **P < .001 compared with the mixture of APL targets and phagocytes (MΦs and ECs separately).
