CD81 controls static integrin adhesion of DCs. Static adhesion of unstimulated immature BM-DCs (A) or of LPS-stimulated (200 ng/mL) BM-DCs (B-C) to surfaces coated with 12.5 μg/mL ICAM-1-FC (A-B) or 50 μg/mL fibronectin (C), respectively. BM-DCs were stimulated with 50 ng/mL PMA. Immunofluorescence stainings reveal that CD81 (red fluorescence) is colocalized with β2 integrins (CD18, green fluorescence) in migration-relevant membrane protrusions (D left panel). PMA stimulation leads to an increased relocalization and strong accumulation of colocalized CD81 and CD18 to the cell periphery at the leading edge of membrane protrusions (D right panel). Error bars indicate ± SD. ***P ≤ .001. Each single experiment was performed in duplicate. Each experiment was repeated at least 3 times independently. Bars represent 10 and 2 μm (magnified detail), respectively.