Mature granulocytes are reduced in bone marrow and peripheral blood after Gabpa deletion. Flow cytometry for B220 and CD11b, forward scatter (FSC) and side scatter (SSC) of bone marrow cells (A; circles indicate cells with a high degree of SSC) and CD11b and Gr1 in bone marrow cells of control and KO mice (B); numbers indicate percentage of cells in individual quadrants or boxes. (C) Wright-Giemsa stained cytospin preparations of the following sorted bone marrow cell populations: control CD11b^hi Gr1^lo cells (open arrows indicate immature granulocytes, and thin arrows indicate monocytic cells), control CD11b^hi Gr1^hi cells, and KO CD11b^int Gr1^lo cells, in left, center, and right panels, respectively. Images shown at ×400 original magnification. All findings include data from at least 3 independent biologic specimens.