Gabpa disruption reduces myeloid progenitors and alters transcription factor expression. (A) Number of in vitro colonies ± SEM per 5 × 104 control and KO bone marrow cells, grown in the indicated cytokines. *P < .03. (B) Wright-Giemsa–stained cells extracted from in vitro colonies of control and KO bone marrow grown in GM-CSF, shown at ×400 original magnification. (C) Flow cytometry for FcγII/III and CD34 of control and KO Lin−, Sca1−, c-kit+ bone marrow cells. Numbers represent percentages of the indicated populations: CMP, GMP, and MEP. (D) Expression of indicated transcription factors by quantitative RT-PCR of control and KO GMP cells normalized to ActinB and expressed relative to control GMP cells. Data are ± SEM. *P < .04. (E) Immunoblotting for Gabpα or β-actin of the indicated tissues from control and KO mice. All findings include data from at least 3 independent biologic specimens.
