The GM-CSF cytokine negatively affects the self-renewal of RUNX1-ETO cells. (A) Representative result of 3 independent replating assays using BM cells transduced with MIP vector (MIP) or MIP-RE (RE), with or without addition of 10 ng/mL of GM-CSF. (B) Differential counts of cytospin samples from colony cells. Representative results of 2 independent experiments are shown. (C) Differential count of CFUs at the first replating. Representative results of 2 independent experiments. CFU-GM indicates granulocyte macrophage CFU; CFU-G, granulocyte CFU; CFU-M, macrophage CFU; CFU-GEMM, granulocyte, erythroid, macrophage, megakaryocyte CFU; and CFU-E, erythroid CFU. (D) Colony-forming efficiency of Kasumi-1 cells infected with MigR1 (MigR1) or MigR1-CSF2RA (CSF2RA) retrovirus. A representative result of average colony numbers of duplicate samples of 2 independent experiments is shown.