Target silencing and oncogene expression are both effective and persistent. (A) Scheme of experimental setup: FACS-sorted Ba/F3 cells expressing pMmiR^Ctrl–BCR-ABL or pMmiR^Raf1–BCR-ABL as shown in Figure 2G, were deprived of IL-3 and either kept in culture or injected intravenously into BALB/c mice. After 2 weeks, cells were reisolated from mouse spleens and cultured for 1 more week. After 4-5 weeks cultured cells or spleen cells from 2 individual mice per group were analyzed for (B) BCR-ABL expression and Raf1 knockdown efficiency by Western blot analysis. (C) The membrane was stripped and reprobed with anti–p-ERK, anti–p-AKT, and anti–p-STAT5 Abs as indicated.