Figure 1
Expression of leukotriene receptor mRNA and protein by human monocyte-derived DCs. (Left panel) DCs were matured for 1 hour with CC (10 ng/mL TNF-α, 1000 U/mL IL-6, 10 ng/mL IL-1β, 1 μg/mL LPS, 12.5 μg/mL pIC, or 1.0 μg/mL recombinant CD40L plus 1.0 μg/mL enhancer). RNA was isolated, reverse transcribed and amplified for 40 cycles using quantitative RT PCR. For comparison, RNA isolated from immature DCs (DC Imm) was also amplified. The data presented are from 1 representative experiments of 3 performed. (Right panel) DCs were matured for 12 hours as indicated and expression of CysLTR1 and CysLTR2 was analyzed by FACS. A representative result for 1 of 3 donors is presented.
