Intracellular calcium mobilization and CysLTR₁ antagonism. (Left panel) DCs were loaded with FluoForte and intracellular calcium release was determined using a Tecan Infinite F200 Pro fluorometer. CD40L was added at t-d and LTC₄ or LTD₄ were injected after 20 seconds at 37°C (arrow) and data were acquired for another 50 seconds. DCs were preincubated with 1 μg/mL PTX or 10μM CysLTR₁ antagonist MK476) for 2 hours before stimulation with CD40L and LTD₄. (Right panel) Peptide-specific CD8+ T-cell responses were induced as described using DC that were matured for 12 hours as indicated in the presence or absence of 10μM CysLTR₁ antagonist MK476. Frequencies of antigen-specific cells were determined in IFN-γ ELISPOT assays. The data are shown as the mean ± SEM of triplicate cultures and represent 1 of 2 experiments that yielded similar results.