FBXL2 and CaM differentially regulate the cell cycle in transformed B lymphoblastoid cells. (A) HCC1739 BL cells were transfected with either control RNA or cyclin D2 siRNA. Forty-eight hours later, cells were labeled with BrdU and analyzed through FACS or stained with annexin V and analyzed using a cell counter (n = 3 experiments, #P < 0.01 versus control (CON) RNA. (B) Cells were preinfected with a replication-deficient Adv-CaM or an empty virus and then transfected with FBXL2 plasmid. Forty-eight hours later, cells were either labeled with BrdU and analyzed through FACS or stained with annexin V and analyzed using a cell counter (n = 3 experiments, *P < .01 versus empty). (C) Cells were cotransfected with FBXL2 with or without 2 cyclin D2^Q98A and cyclin D2^K270R mutants. Forty-eight hours later, cells were either labeled with BrdU and analyzed through FACS or stained with annexin V and analyzed as previously described (n = 3 experiments, *P < .01 versus empty).