FBXL2 and cyclin D2 are differentially expressed in AML and ALL subjects. (A-C) PBMCs from 5 controls, AML, and ALL subjects were cultured in RMPI medium for 18 hours. Cells were then collected, lysed, and assayed for FBXL2 and cyclin D2 by immunoblotting (A). FBXL2 and cyclin D2 protein levels were quantified by densiometry using ImageJ Version 1.45 software, and the distribution of cyclin D2 (B) and FBLX2 protein (C) in each individual subject was graphed (n = 5, P < .005 vs control). (D) Leukemic cells were transfected with FBXL2 using a HVJ-E transfection kit for 24 hours. Cells were stained with annexin V and processed by flow cytometry. Apoptotic cells were quantified and graphed. (n = 5, P < .01 vs control). (E) FBXL2 and cyclin D2 protein levels were also analyzed from representative of AML and ALL transfected PBMCs.