Expression of CLRs on mDC subsets. Expression of CLEC9A, DCIR, DC-SIGN, DEC205, and mannose receptor was analyzed by flow cytometry on day 6 immature monocyte-derived DCs or freshly isolated BDCA1/CD1c+ mDCs, CD16⁺ mDCs, or BDCA3/CD141⁺ mDCs. Representative examples of 3 independent experiments are shown. Filled gray histograms represent isotype controls, open black histograms represent cells stained with specific antibodies. See supplemental Figure 1 for dot plots and gates.