Reactivity of 51p1-encoded Abs isolated from blood of a healthy CMV-seropositive and CMV-seronegative individual with pUL32. Recombinant CMV protein pUL32 and control cell lysate from 293FT cells transfected with pCDNA were size separated on SDS-PAGE and transferred to nitrocellulose membrane. The membrane was probed with CLL 51p1 rAbs and 51p1 rAbs isolated and purified from a healthy CMV-seronegative and CMV-seropositive individual. Secondary Ab alone and CMV-seropositive serum was used as negative and positive control, respectively. All rAbs were tested at the same final concentration (5 μg/mL).