Expression of DAPK2 and PIM1 is enriched in primary MM cells. (A) DAPK2 and PIM1 have high transcript expression in primary MM BMMCs (gray triangles), but not in normal PBMCs (black diamonds) by gene-specific quantitative real-time PCR. BM specimens contained > 95% tumor by immunohistochemistry. DAPK2, but not PIM1, was also highly expressed in normal CD19⁺ B cells (white squares). Bars indicate the median value for transcript expression relative to GAPDH. Statistical significance was determined by 1-sided exact Wilcoxon rank-sum test. (B) Western blotting of cell lysates (20 μg of protein per lane) from normal hematopoietic cells and primary MM BMMCs with Ab against either DAPK2 or PIM1 reveals protein expression in 4 (DAPK2) or 3 (PIM1) of 5 primary MM BMMCs. (C) Western blotting of MM cell line lysates (20 μg of protein per lane) showed expression of DAPK2 and PIM1 in all tested MM cell lines.