NY-ESO-1, MAGE-A3, and MAGE-A4 are widely expressed by primary ATLL cells. (A) Representative results of RT-PCR analysis for mRNA expression of NY-ESO-1, MAGE-A1, MAGE-A3, MAGE-A4, MAGE-A10, CT-7, CT-10, SSX-1, SSX-2, SSX-4, and SCP-1. (B) ATLL samples were subjected to immunohistochemical staining for NY-ESO-1 (E978) mAb and pan-MAGE (57B) mAb. Bar represents 50 μm. These experiments were performed independently at least twice with similar results.