Modulation of miRNAs by INC424/ruxolitinib treatment of SET2 cells. Cells were exposed to INC414/ruxolitinib at 160, 80, and 1600nM for 3 and 6 hours and then processed for miRNA expression analysis by microarray. The concentration of 160nM was selected as the IC₅₀ concentration in a proliferation assay of SET2 cells (not shown). INC424/ruxolitinib caused dose-dependent reduction of the level of phosphorylated STAT5 (measured by FACS) and Pim-1 mRNA (measured by quantitative real-time PCR), indicating effective inhibition of JAK/STAT signaling (top panels). (Bottom panels) Examples of INC424/ruxolitinib-induced miRNA level modulation, as measured using miRNA array, are shown. The levels of hsa-miR-92a-1* and hsa-miR-330-5p dose-dependently decreased, whereas those of has-miR-let-7c increased compared with untreated control cells. Dose effects were statistically significant (P < .05).