RNA-seq reveals the changes in gene expression in response to IL-10 stimulation. (A) Proportion of de novo assembled transcripts corresponding to known genes, novel isoforms, novel genes, and transcript fragments with an ambiguous mapping. (B) Venn diagram showing the overlap of assembled genes with all transcripts from Ensembl. (C) qRT-PCR results of Stat3, Bcl3, Socs3, and Il4ra. PEC macrophages were treated with IL-10 and samples of RNA were harvested after 0 hours (untreated cells), 30 minutes and 1, 2, 4, 8, and 24 hours in the presence of IL-10. (D) FPKM values of selected transcripts for Il4ra, Bcl3, Socs3, Batf, Etv3, Nfil3, Sbno2, and the 5 assembled isoforms of STAT3 found in the RNA-seq data. Error bars are the lower and upper bounds of the 95% confidence interval.