Identification of candidate executors of the AIR by integrating STAT3 ChIP-seq and RNA-seq data. (A) Overlap of STAT3-binding sites within 20 kb of a transcription factor TSS, either up-regulated (red) or down-regulated (blue). (B) STAT3 bound genes (any TSS with a STAT3 binding site within 20 kb are labeled in green). (C) Heatmap showing the up-regulated TFs that have a STAT3 binding site within 20 kb of their TSS. Novel isoforms are marked in red. Fragments or full-length Ensembl transcripts are marked in black. (D) qRT-PCR of 23 TFs up-regulated on IL-10 treatment and with a STAT3 binding site within 20 kb of their TSS. Shown here is a time course of treatment including 0 hours (untreated), 30 minutes, and 1, 2, 4, and 8 hours after treatment. Isoform specific primers were used whenever possible.