DAF expression on polyclonal T cells does not impact their response to allogenic antigen stimulation. Purified polyclonal CD4⁺ (top panels) or CD8⁺ (bottom panels) T cells from normal C57BL/6 WT or Daf^−/− mice were cocultured at different ratios with irradiated splenocytes as APCs from BALB/c mice for 3 days. T-cell proliferation (left panels) was measured by ³H-TdR incorporation and IFN-γ production (right panels) was measured by ELISA. Data are representatives of 3 independent experiments. NS indicates not significant. P > .05.