Splenic DCs from LPS-treated but not naive Daf^−/− mice are more potent T-cell stimulators than similarly treated WT DCs. (A) FACS analysis (left panel) showing that splenic DCs from naive WT but not Daf^−/− mice expressed DAF. There was no difference between splenic DCs from naive WT and Daf^−/− mice in their ability to stimulate OT-II CD4⁺ or OT-I CD8⁺ T cells, as measured by IFN-γ production (right panel). NS indicates not significant. P > .05. (B) FACS analysis (left panel) showing that splenic DCs from LPS-treated WT but not Daf^−/− mice expressed DAF. DCs from LPS-treated Daf^−/− mice were more potent than similarly harvested WT mouse DCs in stimulating OT-II CD4⁺ or OT-I CD8⁺ T cells, as measured by IFN-γ production. *P < .05. All data are representative of 3 independent experiments.