Altered PD-L1 and CD40 expression on peritoneal macrophages and splenic DCs from thioglycollate- or LPS-treated Daf^−/− mice. (A) FACS analysis of CD40 expression on splenic DCs from LPS-treated WT, Daf^−/−, Daf^−/−/C3^−/− or Daf^−/−/C5aR^−/− mice. (B) FACS analysis of CD40 and PD-L1 expression on resident peritoneal macrophages from naive WT or Daf^−/− mice. (C) FACS analysis of CD40 and PD-L1 expression on thioglycollate-elicited peritoneal macrophages from WT, Daf^−/−, Daf^−/−/C3^−/− or Daf^−/−/C5aR^−/− mice. All data are representative of 3 independent experiments.