FL-MSCs are committed to a FRC-like phenotype. (A) Schematic representation of the statistical analysis used to highlight the TNF/LT signature defined as the intersection of the 2 genelists generated by SAM analysis (FC > 2 or < 0.5 and false discovery rate < 0.1%) and paired t test (FC > 2 or < 0.5 and P < .005). (B) Validation by RQ-PCR of the overexpression of TLR4, IL6, HBEGF, IL15, CCL2, IL8, and CCL5 in HD-MSCs stimulated by TNF/LT for 3 days compared with untreated HD-MSCs. Each sample was normalized to GAPDH and compared with expression in untreated HD-MSCs. The results are the mean ± SD obtained on 3 different HD-MSCs. (C) Gene set enrichment analysis enrichment score curves for the TNF/LT signature in MSC samples via use of the SNR statistic to rank the genes. Vertical black lines indicate the position of each of the 811 nonredundant up-regulated genes included in the TNF/LT signature, and the green curve represents the running sum of the weighted enrichment score. (D) Hierarchical clustering of HD-MSCs and FL-MSCs performed on the 1673 PS of the TNF/LT signature. The relative level of gene expression is depicted according to the shown color scale. (E) BL2 cell line was cultured in low serum concentration alone, or with confluent HD-MSCs (n = 9) or FL-MSCs (n = 8), pretreated or not with TNF/LT. Tritiated thymidine (³H-TdR) incorporation was evaluated at day 3. MSCs cultured alone always showed a ³H-TdR incorporation ≤ 500 cpm. **P < .01; ***P < .001