SPR analysis of the influence of PCh on the APC binding to sEPCR. sEPCR was captured on a CM5 chip through RCR-2 mAb. (A) Binding of APC (0, 3, 9, 27, 81, and 243nM) to sEPCR. (B) Binding of APC (0, 9, 27, 81, 243, and 729nM) to delipidated sEPCR. In both cases, a representative experiment of 3 independent repeats is shown. Black lines represent experimental data; and gray lines, fittings to a Langmuir 1:1 kinetic model. (C) Binding at equilibrium of 100nM APC to sEPCR, delipidated sEPCR, and delipidated sEPCR reconstituted with 760 and 786 Da MW PCh. Data are mean ± SD of 3 independent experiments. Mann-Whitney U test was used for statistical comparisons.