Rantes KO HSCs exhibit similar engraftment potential. (A) BM derived from 9-month-old WT or Rantes KO mice was isolated and analyzed for Ly- and My-bi HSCs as follows: KLS cells were identified, followed by the CD150⁺ fraction, followed by analysis of lower and upper SP phenotypes. Representative FACS profiles of lower and upper SPKLS-CD150⁺ HSCs from WT and Rantes KO mice. (B) Overall engraftment of 9-month-old WT or KO HSCs (SP^KLS-CD150⁺) in the PB of recipient mice at 4th, 8th, 12th, and 16th week after transplantation (n = 5 WT, n = 6 KO). (C) Analysis of the BM of recipient mice to determine donor cell contribution to the total stem cell compartment (SP^KLS-CD150⁺) 20 weeks posttransplantation of WT or KO HSCs. (D) Analysis of the BM of recipient mice for the frequencies of donor-derived lower and upper SP^KLS-CD150+ cells. (E) Transplantation of WT HSCs from 18-month-old mice into WT or Rantes KO mice. The graph shows the lineage distribution of donor-derived cells in the WT or KO mice at 12 weeks posttranplantation. Overall engraftment of donor cells is also indicated as (“Engraft”). Error bars represent SEM. Representative of 2 experiments (n = 6 WT, n = 7 KO).