Neutrophils and monocytes/macrophages are necessary for FcγRIIA-dependent PSA. (A-G) Indicated mice were injected with preformed polyclonal IgG-IC (mouse anti-GPI antiserum plus GPI), and central temperatures were monitored. PSA in FcγRIIA transgenic mice injected with (A) anti-Gr1 mAbs (n = 8) or isotype (ISO) control (n = 3), (B) anti–Ly-6G mAbs (n = 4) or untreated control (n = 4), (C) anti-CD200R3 mAbs (n = 3) or untreated control (n = 3). Nontransgenic littermates were used as controls (A, n = 2; B, n = 4; C, n = 3). (D) PSA in W^sh3KOIIA mice and 3KOIIA mice (n = 3). Nontransgenic littermate controls 3KO (n = 3) and W^sh3KO mice (n = 4) were used as controls. (E-F) PSA in 3KOIIA mice injected with (E) PBS liposomes (PBS lipo) or clodronate liposomes (toxic lipo; n = 3), (F) Gadolinium chloride (GdCl3) or not (n = 4). Nontransgenic littermates were used as controls (E, n = 2; F: n = 4). (G) PSA in 3KOIIA mice left untreated (n = 7), or injected with anti-Gr1 mAbs (n = 5), toxic liposomes (n = 6) or anti-Gr1 mAbs plus toxic liposomes (n = 6). Data are a compilation of 2 experiments. 3KO served as negative control (n = 2). Statistical significances are indicated among 3KOIIA groups. (H) mAb IV.3-induced PSA in indicated mice injected with anti-Gr1 mAbs, toxic liposomes, or left untreated (n = 3). (A-H) Data are represented as mean ± SEM, and (A-F,H) are representative of at least 2 independent experiments (*P < .05; **P < .01; ***P < .001).