Expression of majority of membrane proteins was reduced in V205GR erythrocytes. Coomassie brilliant blue-stained SDS-PAGE profile (A) and relative immunoblotting expression levels of erythroid membrane proteins (B) in red blood cell ghosts. Three WT and 3 V205GR-A newborn pups were used for this experiment. Loading volume was adjusted by the original number of erythrocytes used for preparation of cell ghosts.