Figure 1
Figure 1. ArhGAP15 regulates Rac activity in phagocytes. (A) Quantitative RT-PCR analysis of ArhGAP15 expression in spleens derived from ArhGAP15+/+ and ArhGAP15−/− mice; n = 3/genotype. (B) Representative detection of ArhGAP15 Western blot from macrophage (MØ) and neutrophils (NØ) of ArhGAP15+/+ (WT) and ArhGAP15−/− (KO) mice. Only the lower band, exclusively present in WT lanes, corresponds to the correct size of ArhGAP15. Equal loading was monitored by vinculin expression; n = 3. (C-F) Determination of Rac1 activation after C5a stimulation in macrophages (C-D) and neutrophils (E-F). Representative detection of active (Rac1-guanosine triphosphate) and total Rac1 is shown. Normalization was obtained by setting the percentage stimulation of ArhGAP15+/+ cells after 1 minute for macrophages and after 5 seconds for neutrophils to 100%; at each point, n = 8/genotype. #P < .05. **P < .001.

ArhGAP15 regulates Rac activity in phagocytes. (A) Quantitative RT-PCR analysis of ArhGAP15 expression in spleens derived from ArhGAP15+/+ and ArhGAP15−/− mice; n = 3/genotype. (B) Representative detection of ArhGAP15 Western blot from macrophage (MØ) and neutrophils (NØ) of ArhGAP15+/+ (WT) and ArhGAP15−/− (KO) mice. Only the lower band, exclusively present in WT lanes, corresponds to the correct size of ArhGAP15. Equal loading was monitored by vinculin expression; n = 3. (C-F) Determination of Rac1 activation after C5a stimulation in macrophages (C-D) and neutrophils (E-F). Representative detection of active (Rac1-guanosine triphosphate) and total Rac1 is shown. Normalization was obtained by setting the percentage stimulation of ArhGAP15+/+ cells after 1 minute for macrophages and after 5 seconds for neutrophils to 100%; at each point, n = 8/genotype. #P < .05. **P < .001.

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