Loss of ArhGAP15 affects macrophage morphology but not migration. (A) Representative images of ArhGAP15^+/+ and ArhGAP15^−/− BMDMs were stimulated with C5a and stained for filamentous actin (red) and nuclei (blue). (B-C) Perimeter analysis and elongation ratio of ArhGAP15^+/+ and ArhGAP15^−/− BMDMs before and after C5a stimulation (n ≥ 60 cells/genotype). (D) Boyden chamber chemotaxis and chemokinesis analysis of ArhGAP15^+/+ and ArhGAP15^−/− BMDMs; n = 3/genotype. #P < .05. *P < .01. **P < .001.