Effects of RhoJ on tube formation in Matrigel in vivo. Matrigel mixture containing 2μM control or RhoJ siRNA was injected subcutaneously into nude mice. The gel plugs were collected after 7 days. After fixation in paraffin, samples were embedded in paraffin, sectioned and H&E stained. (A) Representative images from H&E staining. (B) Representative images of immunohistochemistry staining using CD31 antibody. (C) The quantitative data are shown as the number of vessels containing red blood cells relative to the control condition (n = 5).