Partial rescue of ERG siRNA effects on EC lumen and tube formation by adenoviral delivery of RhoJ. (A-B) HUVECs were first transfected with ERG, RhoJ, or control siRNA (40 nmol/L) before infection with adenovirus encoding either GFP or RhoJ as indicated. ECs were then seeded in 3D collagen matrices for 24 hours. Lumen area was quantitated using Metamorph software and is presented relative to lumen area obtained for the control GFP adenovirus. *Comparison with CTR siRNA-treated EC. **Comparison with Ad-GFP infected EC with the same siRNA treatment. (C) ECs were infected with adenovirus encoding either GFP or RhoJ. The expression of GFP-RhoJ fusion protein was detected by Western blot using anti-RhoJ antibody and actin as a loading control.