Phenotypic and functional characterization of NCR⁺ Vδ1 T cells producing cc-chemokines. (A) Summary graphs of statistical dot plots showing the percentages of freshly purified (day 0) and in vitro activated (day 14) Vδ1 T cells expressing NKp30, NKp46, NKp44, and NKG2D. (B) Levels of CCL3, CCL4, CCL5, and CXCL12 spontaneously secreted by Vδ1 T cells (white bars) compared with those of Vδ1 T cells whose NKp30 (red bars), NKp46 (blue bars), NKp44 (green bars), NKG2D (brown bars), or TCR-γδ (black bars) were previously cross-linked with anti-NKp46, anti-NKp30, anti-NKp44, anti-NKG2D, and anti–TCR-γδ mAbs, respectively. Data are presented as a mean of 10 independent experiments (with P values and SD) performed in duplicates from 10 unrelated healthy donors. *P > .0001. #P < .05. Differences were assessed using the Mann-Whitney test, and all P values are 2-sided and unadjusted. n.s. indicates not significant.