Suppression of HIV-1 replication through cc-chemokines secreted on NKp30 engagement on Vδ1 T cells. (A) Time course experiments showing the levels of viral replication in PM1 cell line infected with HIV-1BaL R5 strain either in the absence (yellow bars) or in the presence of supernatant from cultures of Vδ1 T cells whose NKp30 (red bars), NKp46 (blue bars), NKp44 (green bars), NKG2D (brown bars), and TCR-γδ (black bars) were previously cross-linked with anti-NKp46, anti-NKp30, anti-NKp44, anti-NKG2D, and anti–TCR-γδ mAbs, respectively. *P > .0001. #P < .05. Differences were assessed using the Mann-Whitney test, and all P values are 2-sided and unadjusted. n.s. indicates not significant. (B) The detection of HIV-1 replication in PM1 cells line (yellow bars) was performed either in the absence (red and black bars) or in the presence (pink and gray bars) of neutralizing mAbs to CCL3, CCL4, and CCL5. Data are presented as a mean of 10 independent experiments (with P values and SD) performed in duplicates from 10 unrelated healthy donors.
