The NE reuptake inhibitor desipramine enhances G-CSF–triggered HSC/progenitor mobilization. (A) Treatment scheme of 8-week-old male C57BL/6 mice for HSPC mobilization using G-CSF in combination with desipramine. (B-C) Absolute numbers of CFU-Cs (B) or LSKF cells (C) in the peripheral blood of mice mobilized with G-CSF alone (G) or in combination with desipramine (G+D), respectively (n = 4-5). (D) Gating strategy and representative dot plots to determine LSKF numbers in the peripheral blood of mice mobilized with G-CSF alone or in combination with desipramine, respectively. (E) Long-term culture-initiating cells per milliliter in the blood of mice mobilized as in panel A (n = 4-5). (F) Experimental design to assess the effect of desipramine on G-CSF–induced mobilization in poor mobilizer mice. (G) LSKF cells per femur (n = 12-14) in poor mobilizer mice. (H-I) CFU-Cs (H) and LSKF cells (I) after mobilization in the peripheral blood of poor mobilizer mice (n = 8-7). Dashed lines indicate the mobilization values in nonirradiated mice as determined in panels B and C. *P < .05; **P < .005.