Figure 2
Figure 2. Effects of panobinostat and everolimus (RAD001) on mTOR activity. (A) Cells were incubated with diluent control or LBH589 (0.02-0.1μM); and after 48 hours, cell lysates were prepared and resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with (A) anti–phospho-AKT (Ser473), anti-AKT, anti–phospho-p70S6K, anti-p70S6K, anti–phospho-4EBP1, anti-4EBP1, and anti–β-actin antibodies. Representative blots from 3 independents experiments are shown. (B) Cells were incubated with diluent control or RAD (0-5μM); and after 48 hours, cell lysates were prepared and resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti–phospho-AKT (Ser473) anti-AKT, anti–phospho-p70S6K, anti-p70S6K, anti-pS6 ribosomal, and anti–β-actin antibodies. Representative blots from 3 independents experiments are shown.

Effects of panobinostat and everolimus (RAD001) on mTOR activity. (A) Cells were incubated with diluent control or LBH589 (0.02-0.1μM); and after 48 hours, cell lysates were prepared and resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with (A) anti–phospho-AKT (Ser473), anti-AKT, anti–phospho-p70S6K, anti-p70S6K, anti–phospho-4EBP1, anti-4EBP1, and anti–β-actin antibodies. Representative blots from 3 independents experiments are shown. (B) Cells were incubated with diluent control or RAD (0-5μM); and after 48 hours, cell lysates were prepared and resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti–phospho-AKT (Ser473) anti-AKT, anti–phospho-p70S6K, anti-p70S6K, anti-pS6 ribosomal, and anti–β-actin antibodies. Representative blots from 3 independents experiments are shown.

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