immNK cells display an unique pattern of chemokine and cytokine release. UCB CD34⁺ cells were purified and cultured in the presence of cytokine-mix for 20 days. At this time interval, we purified by cell sorting: (A) immNK cells (CD161⁺CD56⁺LFA-1⁻), (B) difNK cells (CD161⁺CD56⁺LFA-1⁺), and (C) myelomonocytic cell precursors (CD33⁺CD161⁻CD56⁻LFA-1⁺). Purified cells were further cultured in the presence of cytokine-mix and after 72 hours of culture supernatants were collected and analyzed by ELISA multiplex assay for the indicated cytokines and chemokines. Data are expressed as mean (± SEM) of pg/mL obtained in 3 independent experiments.