CXCL8 release is induced by CD161 and NKp44 cross-linking. Purified CD161⁺CD56⁺LFA-1⁻ immNK cells were incubated in GAM-coated plates in the presence of RPMI+15% serum (medium), cytokine-mix, anti-CD161, or anti-NKp44 mAbs. After 24 hours, supernatants were collected and analyzed for the indicated cytokines and chemokines by ELISA multiplex assay. Data are expressed as mean (± SEM) of pg/mL obtained in 3 independent experiments.