Activation of PTH signaling in osteocytes does not change BM HSC function. (A) Schematic representation of the competitive reconstitution experiments. Donor BMMCs were collected by crushing the hind limbs (1 femur and 1 tibia per each donor mouse) from CD45.2 WT or TG mice, mixed with CD45.1 competitor cells at a ratio of 1:2, and transplanted into 4-5 irradiated CD45.1 recipient mice to quantify engraftment potential from individual donors. (B) Representative flow cytometric dot plot of total cells from peripheral blood of donor illustrates how engraftment was quantified. Donor cells are in the right lower quadrant (CD45.2⁺/CD45.1⁻). Individual recipients are represented as dots (to see this information segregated by donor, see supplemental Figure 2). (C) Results of the competitive repopulation assay. The percentage of CD45.2⁺/CD45.1⁻–expressing total cells, myeloid cells (CD11b⁺), T cells (CD3ϵ⁺), and B cells (B220⁺) in the blood of recipient mice was analyzed at 4, 8, 12, and 16 weeks after transplantation as indicated. Each dot represents an individual recipient mouse. Bars indicate the mean and SEM.